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Author: Admin | 2025-04-28
Site UB but a sharp signal at site AB (at site UB this could be a mixture of some RNA mononucleotides and β-glycerophosphate (D = within δ 4.1 to 3.9), RNA mononucleotides (E = δ 3.81, 3.74 and 3.68 ppm), an unknown phosphomonoester (F = δ 3.5 ppm), and broad phosphomonoesters present underneath some of the sharp signals (G = within δ 4.8 to 3.4 ppm). Sample materials for Site 26 years, Site UB and Site AB were diluted with 2.5, 2.0 and 1.5 times more NaOH-EDTA than all other sites (years 2 to 20), respectively, due to high sample viscosityFull size imageThe chemical nature of phosphomonoesters at site UB was different to that at site AB, but similar to the soils along the 26-year chronosequence (Fig. 3). The chemical nature of phosphomonoesters within the 26-year chronosequence was generally the same, except that at the beginning (site year 2), which had less signal.The concentration of organic P (phosphomonoesters and phosphodiesters) in NaOH-EDTA extracts as determined by NMR spectroscopy was 7.2 mg kg−1 at year 2 (Table 4). The concentration of organic P increased along the 26-year chronosequence but remained relatively constant with an average of 15 mg kg−1, which was similar to site UB (Table 3). Phosphomonoesters were the dominant class of organic P across all soils, which were on average 82% of the organic P. In general, concentrations of pyrophosphate were low (−1) but noticeably higher than those of phosphodiesters up to 13 years of restoration.Table 4 Concentrations (mg kg−1) of P classes in NaOH-EDTA extracts (1:4 soil to solution ratio) of soils (0–4 cm) along the chronosequence of restored sites, a long unburned native forest site (UB), and an unmined, previously burned native forest site (AB) (sampled October 2002)Full size tableDiscussionWe hypothesised that the initial phase of successful
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